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The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock

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SH-SY5Y and BV-2 cells were grown in 6-well plates at densities of 1 106 and 5 105 cells/well, respectively

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MEDI7247 is the only ASCT2 antibody-drug conjugate currently in phase I clinical trials (NCT03811652, NCT03106428)

Small clinical studies have reported improvements in melanin index or selected skin properties after oral glutathione use