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Children with GSTM1 null or GSTP1 val/val genotypes had increased respiratory symptoms upon exposure to ozone [33, 57, 58]

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Briefly, metabolites in plasma (100 L) were extracted with 90% methanol (900 L), and proteins were precipitated at -20 C for 2 h, followed by centrifugation for 10 min at 4000 rpm at 4 C

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193 Homocysteine

A sterile syringe equipped with a 2125 gauge needle was inserted into the left ventricle through the chest wall to aspirate blood