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Live bacteria were selected in the yield mode with an appropriate FSC/SSC gate while setting a sorting gate to collect 25 % of those cells with the highest sfGFP fluorescence (4 10 7 events in total)

Source: ResearchGate Glucocorticoid Signaling Glucocorticoids, secreted primarily by the adrenal glands, are important clinical drugs used to control pain and reduce inflammation in autoimmune diseases, neurological injury, cancer, and more

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for 10 min, 25 L of agonist was dispensed into the well using a FlexStation III microplate reader (Molecular Devices), and the intracellular calcium change was recorded at an excitation wavelength of 485 nm and an emission wavelength of 525 nm