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Proinflammatory cytokine-induced alpha cell impairment in human islet microtissues is partially restored by dual incretin receptor agonism

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Cells were then labelled for intracellular marker FOXP3/ AlexaFlour680 (Cat# HCA116A647, Bio-rad) antibody, diluted in permeabilization buffer

Biochemical results indicated that the protein levels of ATF6, IRE1, and PERK were drastically increased following CSD (Figure 5A)