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It is also required in the production of DNA, which is a genetic material that is present in all human cells

5 l of metabolites suspension was loaded and resolved on an Acquity UPLC BEH C18 column (1.7 m, 2.1 100 mm, Waters) using mobile phases of water with 0.1% formic acid (buffer A) and acetonitrile with 0.1% formic acid (buffer B) with a flow rate of 0.3 ml/min and 8 minutes long gradient

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With this aim, C6 cells were grown in 24-well cell culture plates until 80% cell confluence was reached

Exp Mol Med 50 , 116 (2018)

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