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To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol

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4,54 In addition to the RING E3 ligase Pirh2, the WW-domain, HECT-containing, E3 ligase Itch/AIP4 bound within aa 397571 of the SAM domain of p63 and induced its ubiquitination and degradation