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Zhang Q, Sun T, Yu F, Liu W, Gao J, Chen J

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fold change) were extracted from primary experimental studies across C

The genes for human IgGl and IgG4 heavy chain constant regions {IGHG1 and IGHG44) are amplified using PCR primers containing an Ascl and a Kpnl site ( : GAT CGG TAC CGA TCG GCG CGC CCA AAT CTT GTG ACA AAA CT CAC (SEQ ID NO:54), 4: GAT CGG CGC GCC TTC CAC CAA GGG CCC ATC CGT CTT CCC CCT (SEQ ID NO: 55)) and a Sfil site ( : GAT CGG CCC AGC CGG CCT CAT TTA CCC GGA GAC AGG GAG AGG CTC TTC (SEQ ID NO: 56), 4: GAT CGG CCC AGC CGG CCT CAT TTA CCC AGA GAC AGG GA(SEQ ID NO: 57)), the C H 2-3 and 4 C H 2-3 domains using primers containing an Ascl and a Kpnl site ( : GAT CTC TAG ATC ATT TAC CCG GAG AC A GGG AGA GGC TCT TC (SEQ ID NO:58), : GAT CGG CGC GCC CAG CAA CAC CAA GGT GGA CA (SEQ ID NO:59)) and a Xbal site ( : GAT CGG CGC GCC AGC CTC CAC CAA GGG CCC AT (SEQ ID NO: 60), : GAT CTC TAG ATC ATT TAC CCA GAG ACA GGG A )

The decision in the amount of animals used for the study was following the 3Rs (Replacement, Reduction and Refinement) to avoid unnecessary sacrifice