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specifically, semaglutide is a GLP-1 receptor agonist with 94% sequence homology to human GLP-1

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The beads were washed 3 times with cold homogenization buffer B to remove non-specifically bound proteins, by gentle centrifugation at 2000 x g for 10 seconds at 4 C in a table-top centrifuge The bead pellet was resuspended in SDS-PAGE sample buffer and heated at 100 C for 10 mins before analysis by SDS-PAGE