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After incubation, neutrophils were washed and stained with antibodies targeting CD11b (1:500 dilution, 101224, BioLegend) and Ly6G (1:500 dilution, 127608, BioLegend)

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Post transfer, membranes were stained with amido black (0.1% w/v Amido black, 10% v/v acetic acid and 25% v/v isopropanol) to monitor protein transfer, then de-stained 10% v/v acetic acid and 25% v/v isopropanol

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612 NMN treatment improves the neuronal functions, including rescuing the memory and cognition in rodent models for Alzheimers disease, protecting neurons from cell death after intracerebral hemorrhage or ischemia, recovering severe retinal degeneration and restoring the age-associated loss of the neural stem/progenitor pool

10.1056/EVIDoa2300332 NEJM Evid