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The A-side buffer contained 100 M Lucifer yellow dye, in transport buffer (1.98 g/L glucose in 10 mM HEPES, 1 Hank's balanced salt solution, pH 7.4), and the B-side buffer was transport buffer at pH 7.4

After the first two months, it is common to see 5-10% of their initial body weight

Two primary tests measure the amount of B12 in blood (low in B12 deficiency): Total B12 test: measures total amount of B12 Active B12 test: measures amount of B12 that is available to the cells Two secondary tests measure function of B12 in the cell (raised in B12 deficiency): MMA: measures function of B12 in the mitochondria Homocysteine: measures function of B12 for methylation How does Vitamin B12 deficiency get diagnosed

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Ex vivo cytotoxicity assay NIH/3T3 cells, transfected with FAP protein, were used as target cells

High prevalence of cobalamin deficiency in elderly outpatients