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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

Autologous mitochondrial transfer as a complementary technique to intracytoplasmic sperm injection to improve embryo quality in patients undergoing in vitro fertilization-a randomized pilot study

Hochholdinger et al., 2018)

Indeed, levels of Gclc transcripts and reduced glutathione (GSH) increased upon activation B6 FoB in vitro (Fig

In contrast, knocking out of TXNIP limited the production of lipid ROS and cellular ROS triggered by RSL3 + JQ1 (Figs

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