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the PCR amplicon sizes were used to check for the presence of the insertion and the strain was crossed with wild-type worms to remove the dpy-10 coinjection marker

This does not mean the concern is irrelevant

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Nutrients 13:3432 Stpie M, Stpie A, Wlaze RN, Paradowski M, Banach M, Rysz J (2014) Obesity indices and inflammatory markers in obese non-diabetic normo-and hypertensive patients: a comparative pilot study

Mechanistically, acetyl-CoA, a derivative of BCKAs, inhibits the interaction between PR domain-containing protein 16 (PRDM16) and peroxisome proliferator-activated receptor- (PPAR-) by acetylating the k915 site of PRDM16 to maintain WAT characteristics