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The xenograft mice were treated with 5-FU alone, RSL3 alone, or a combination of 5-FU and RSL3 ( Figure 5E )

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John Dick's group had originally identified the AML LSCs in primary patients using serial xenotransplantation experiments in NOD/SCID immunodeficient mice and observed that LSCs reside in lineage-CD34 + CD38 cells fraction ( + cells enriches the LSCs frequency ( AML LSCs and blasts reside in bone marrow (BM) niches, where the nutrients are limited