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Bilirubin as a potent antioxidant suppresses experimental autoimmune encephalomyelitis: implications for the role of oxidative stress in the development of multiple sclerosis

Both buffer and protein solution were pre-equilibrated for 1 h at the temperature of the subsequent HDX experiment (20 C)

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and step 3: stirring to dissolve and clarify the prepared solution, adding water for injection to full amount, and adjusting pH to 7.0 with 10% sodium hydroxide solution

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The biomarker results of the ECLIPSE study were reviewed by Faner et al