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For the determination of mitochondrial swelling, 100 g isolated mitochondria in 200 l isolation buffer were used

Additionally, we analyzed genome-wide distributions of Tbp1(TATA-box binding protein, TBP) and the Rad21 cohesin subunit because we previously found that Tbp1 interacts with the Cnd2 condensin subunit and that cohesin and condensin both tend to localize at the 3 ends of Pol II genes 34,49

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doi: 10.1016/s0361-3682(01)00052-6 45

We obtained fibroblasts from: (i) a 34 years-old non-carrier female donor (Familial CTRL), (ii) a 34 years-old TDP-43 G376D carrier female donor who was asymptomatic at the time of biopsy and is still asymptomatic 5 years later