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doi: 10.1016/j.pharmthera.2019.107396 40 ShenYLuCSongZQiaoCWangJChenJet al

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[30] verified that HIF-1-BNIP3-mediated mitophagy functioned as a protective mechanism for acute kidney damage by inhibiting apoptosis and ROS production

Briefly, L-lysine (0.78 g, 5 mmol) and D, L-Hcy thiolactone hydrochloride (0.93 g, 5 mmol) were dissolved in 100 mL 0.2 M sodium phosphate buffer, pH 7.4, 0.2 mM EDTA and allowed to react for 24 h at room temperature

Shiloh Y and Kastan MB