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Proteins were separated by electrophoresis on 7.5%, 10% or 12% polyacrylamide gel (depending on the molecular weight of protein), and then electrophoretically transferred to a polyvinylidene difluoride (PVDF) membrane

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Cystic Fibrosis Cystic fibrosis is an autosomal recessive disorder caused by a mutation in the CF transmembrane conductance regulator (CFTR) gene encoding the CFTR protein which regulates the movement of chloride and sodium ions across epithelial cell membranes