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The intrinsic pathway can be triggered either as a result of mPTP opening, or activation of the proapoptotic proteins of the B-cell lymphoma 2 (Bcl-2) family, Bax and Bak [192] after the removal of the block of antiapoptotic proteins Bcl-2 and Bcl-XL [193]

doi:10.1046/j.1365-2125.2001.01305.x 104

Then, it was subjected to 5 min of bath sonication to break up any large residual molecules further

10.1016/j.freeradbiomed.2018.12.011 107

To fully understand this, one needs to consider the mechanism of the methionine synthase reaction in relation to vitamin B12 and its various oxidation states