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Fresh versus cryopreserved biopsy scRNA-seq comparison For the fresh versus cryopreserved comparison, twelve colon biopsies were divided into two vials, each containing six biopsies, for two donors

IDO1 activity accelerates Trp depletion and upregulates the expression of WRS through the general control non-derepressible-2 kinase (GCN2)/phosphorylated eukaryotic translation initiation factor 2 (peIF2)/activating transcription factor 4 (ATF4) signaling pathway (Liu et al., 2016)

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In particular, short chain fatty acids produced by gut bacteria, are capable of affecting gut permeability by strengthening tight junctions, stimulating mucus production and IgA synthesis (16)

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