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Molecules with an unpaired electron are reactive and look for another molecule with an unpaired electron to bind to

7.2 Biomarker translation: from association to utility The journey of circulating IGFBPs into clinical tools demands a rigorous, multi-stage process: Analytical Validation: The initial step requires developing standardized, reproducible assays for candidates (e.g., IGFBP-2, -4, -7) that meet regulatory guidelines for precision, accuracy, and specificity

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In addition, we also found a significant increase in the -hydroxybutyrate/acetoacetate ratio in HFD that reflects higher BOH levels in the plasma of HFD mice ( Fig