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Optimization of the best extender for liquid semen of Muscovy duck (Cairina moschata) with the addition of different concentrations of vitamin C and glutathione

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Quantification of protein levels by ELISA To accurately measure the hepatic concentrations of diverse proteins, encompassing the apoptotic regulators Bax and Bcl-2, as well as the principal inflammatory mediators TNF-, IL-6, NLRP3, and IL-1, the highly sensitive ELISA technique was utilized, employing specific commercial kits