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The reaction was initiated by mixing 2035 g protein with a reaction mixture containing 10 mM ATP, 5 mM L-cysteine, 50 mM L-glutamate, 500 M acivicin, 20 mM MgCl 2 , 100 mM Tris-HCl pH 8.2 in a total volume of 150 l followed by incubation for 15 min at 25C

"Graduate School of Medical Sciences | Weill Cornell Medicine"

6 Its a vicious cycle, unfortunately

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doi: 10.1159/000360101 154

Acknowledgements The authors are supported by National Institutes of Health (NIH) grants R33HL154252, R01EY035853 and U54CA268083 (P.C.S.), and VA RSF Banks.025 (W.A.B.)