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Science 2020, 368 (6486), 7884

Starting with single additions allows users to validate value before committing to more complex and costly protocols

doi: 10.3390/nu10040440 46

(c) denaturing the resulting single- or double-stranded oligonucleotides to produce a mixture of single-stranded polynucleotides, optionally separating the shorter or smaller polynucleotides into pools of polynucleotides having various lengths and further optionally subjecting said polynucleotides to a PCR procedure to amplify one or more oligonucleotides comprised by at least one of said polynucleotide pools