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2011 Oct 1;18(29):4454-69

PLoS One 17:e0262937

Determination of toxic and essential trace elements in serum of healthy and hypothyroid respondents by Icp-Ms: a chemometric approach for discrimination of hypothyroidism

High resolution purification of GST-tagged proteins

JL and YL assisted in preparing figures and tables and participated in manuscript editing

After immobilizing the fusion protein on Ni 2+ -NTA beads, on-column SaSrtA-mediated self-cleavage in the presence of Ca 2+ is done by cleaving the Thr-Gly peptide bond of the sortase recognition motif Leu-Pro-Glu-Thr-Gly (LPETG)