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(8) Matthew G Engel, Sushma Narayan, Min-Hui Cui, Craig A Branch, et al (2025) Intranasal long R3 insulin-like growth factor-1 treatment promotes amyloid plaque remodeling in cerebral cortex but fails to preserve cognitive function in male 5XFAD mice Journal of Alzheimers Disease, 2025 Jan, Volume 103 (Issue 1), Pages 113-126

For excess iron treatment, 48 h after haem depletion, the medium was replaced with HD medium containing 1 mM ferric ammonium citrate (HD + FAC) or 2 mg ml 1 holo-transferrin (HD + holo-Tf) for an additional 24 h

Cell cultures Primary mouse brain endothelial cell cultures were established from 7 days old mice or rats

coli produces colibactin, a secondary metabolite synthesized by the pks genomic island

Short and hydrophobic groups such as the acetyl moiety fit optimally into the narrow pocket, supporting highly efficient catalysis