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It is not the most hands-on intensive protocol, so usually you can read a paper or design a new plasmid as you clean your beads in the background

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Furthermore, primer design for RT-qPCR experiments (Table S3) was executed using Primer Premier software (version 5.0), and primer specificity was confirmed using the Primer Check function of TBtools-II software

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Well, this was not an ED specific study and as such, does not necessarily apply to the ED management of undifferentiated patients but its important to recognize that the existing literature does not support this practice