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In the previous study, food-derived glutathione disulfide was, however, detected in liver and blood red cell of mice

Cell culture The human colorectal carcinoma cell lines HCT116, SW403, HT29, and SW620, human breast adenocarcinoma cell lines MCF-7, SK-BR-3, and MDA-MB-231, and the human embryonic kidney 293T (HEK293T) cells were obtained through the American Type Culture Collection (ATCC, Manassas, VA, USA)

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[cited 3 May 2023]

Cells were stained for viability and surface markers, fixed with 3.7% formaldehyde (Sigma), permeabilized with 0.1% NP-40 and stained for intracellular cytokines

To increase energy expenditure, caffeine and other coffee compounds, such as diterpenes, can reduce the risk of obesity and loss of muscle mass