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Distinct regulatory profile compared to sedative agents: Conventional sedatives typically enhance inhibitory neurotransmission directly, often suppressing neural activity broadly

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After extensive washing, samples were eluted with lysis buffer supplemented with 500 mM imidazole and loaded onto a HiLoad 26/600 Superdex 75 pg column (GE Healthcare) equilibrated in 25 mM sodium phosphate pH 6.7 and 150 mM NaCl