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In contrast, in IFN-R / mice cuprizone-induced microglia accumulation was initially diminished or simply delayed (Mana et al., 2006) that was reflected at week 3 of cuprizone diet by a reduced expression of microglia-associated molecules such as IGF-1 and TNF- that have been proposed to promote proliferation of OPC

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Pressing an ice pack against the injection site for 30 to 60 seconds before injecting constricts local blood vessels, reducing the chance of bruising and potentially dampening the initial immune response