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Ultimately the plasmids have to be transported into the nucleus via the nuclear pore complex (NPC) which forms an aqueous channel through the nuclear envelope and it was estimated that about 0.1% of plasmids are able to enter the nucleus from the cytosol

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The samples were then vortexed and shaken to ensure thorough mixing and subjected to centrifugation at 7000 g for one minute, and the resulting supernatants from the three centrifugations were combined and dried at 40 C for a period of one night

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