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Membranes were then blocked in Starting Block T20 solution (Thermo Fisher Scientific) at room temperature for 1 hour, washed 3 times with PBS with Tween-20 (PBST), and incubated with an appropriate dilution of the primary antibody (all antibodies were used at a dilution of 1:1,000 except for GAPDH and -actin, which were used at dilutions of 1:5,000) in Starting Block T20 solution overnight at 4C

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[DOI] [PubMed] [Google Scholar] 39.Murrel G.A.C

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In another study, the sum of HPMA and CEMA excreted in the urine during the first 24 h after intraperitoneal administration of acrolein to rats (0.5-2.0 mg/kg) accounted for 29.2 6.5% of the dose