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Environ Sci Technol 51:1364913658 Wang L, Xu J, Zhao PY, Pan CP (2011) Dissipation and residues of fluroxypyr-meptyl in rice and environment

Segev, Mara A

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doi: 10.1186/s13045-019-0720-y 176 WeiJWangZWangWLiuXWanJYuanYet al

To purify K29/K48-Ub8, we mixed the reaction solution with four volumes of 50 mM ammonium acetate buffer (pH 4.5) and loaded them onto a Resource S cation exchange column pre-equilibrated with the same buffer

TMPK and CMPK activity assay by NADH-coupled enzymatic assay Activity assay was measured using the spectrophotometric method by coupling ADP formation to NADH oxidation catalyzed by pyruvate kinase and lactate dehydrogenase as described previously 20