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Non-canonical independent defense systems In addition to the canonical GPX4/GSH axis, cells possess other parallel, GPX4-independent anti-ferroptotic pathways that provide additional layers of protection

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Lysates were diluted to final concentrations of 200 mM NaCl, 0.5% NP-40, and 0.05% Na-deoxycholate, and incubated with GFP-binding protein (GBP) beads for 2 h at 4 C

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