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What does getting a B12 injection actually feel like

It may reduce GI discomfort, support connective tissue health during increased training, and provide systemic anti-inflammatory benefits

Split dosing may help if: single morning dose produces effects that fade by afternoon, you are using higher doses (450-500mcg) where sustained levels may help, or evening administration fits your schedule better than early morning

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B12 deficiency has a small number of common causes worth recognising before reaching for an ampoule

The commonly used method is high-performance liquid chromatography, supplemented by other validation methods, as detailed below