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Together, these G4-and nucleic acid-targeted approaches represent a paradigm shift from broad nuclear localization to molecularly precise damage, with successive advancements significantly improving PDT efficacy and reducing systemic toxicity. 6 Targeting cell membrane for enhanced PDT Due to ROS possess a short half-life and a restricted effective diffusion radius, the introduction of photosensitizers to produce ROS in situ on target organelles is essential to avoid their rapid decay

The blend was then heated in a boiling water bath at 100C for 15 min