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Collectively, it seems that MeCbl is different from other previously identified Cys-reactive compounds, particularly the nonspecific GSDME inhibitor DMF, most of which lack Cys residue specificity

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Metabolomics analysis by LC-MS LC-MS/MS analysis was performed using a Thermo Scientific Dionex UltiMate 3,000 UHPLC system coupled to a Thermo Orbitrap Fusion Lumos mass spectrometer