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The purpose of this study was to investigate if there is a correlation between pruritus relief and opioid antagonist PK

After blocking with bovine serum albumin, the membranes were washed three times using tris-buffered saline with Tween 20 and incubated overnight with specific primary antibodies, followed by incubation with the corresponding secondary antibodies

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Large-scale randomized controlled trials (RCTs) are urgently needed to validate these approaches