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Briefly, pinocytic cell loading reagent (Influx, Life Technologies) was prepared according to manufacturers instructions by heating reagent vials for 5 min to melt the PEG cap using an 80 C water bath, adding 4.7 ml of prewarmed culture medium (without serum) per vial, mixing vigorously at 37 C for 30 min to dissolve sucrose crystals, and then adding FBS (5% v/v) and sterile HEPES (10 mM)

This is one of the principles covered in our guide to common peptide storage mistakes using questionable BAC water is on that list for good reason

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10.1038/ejcn.2017.58 69

Wright et al., 2011)

Multivitamins with B12 as part of a number of other necessary nutrients