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The fragment was digested by Nde I and Xho I and inserted into Nde I- Xho I-treated pET28a, to generate recombinant plasmid pET28a- gr

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At the end of storage, the POD activities of 4MIC treated fruit were 1.29-fold than the control (Figure 8B)

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More importantly, the reperfusion or restoration of blood flow after ischemia can also induce the increase of ROS and lipid peroxidation, leading to tissue neuronal damage and worsening of the inflammatory response