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Taken together, these results thus show that Cys4p activity is reduced in the presence of Mck1p but increased in the absence of Mck1p, suggesting that Mck1p inhibits directly or not Cys4p activity, and that Mck1p kinase activity is required

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In many systems, the affinity ligand is directly attached to the bead

Starting conservatively and adjusting based on observed effects represents the most prudent approach given limited clinical guidance

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