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The specific methodology involved the following steps: (1) Retrieve the high-resolution structures of the Tier 1 target proteins from the Protein Data Bank (PDB), import them into PyMOL, remove solvent molecules and small organic molecules, and add hydrogen atoms and export the structures in PDBQT format

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If earlier studies on twins suggested heritability as high as 8090% for ASD with little contribution from the environment [26], according to recent evidence, up to 4050% of variance in ASD liability is determined by environmental factors [27,28]

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however, since mTOR is also described as a nutrient stress sensor, it is difficult to determine whether this effect is due to direct targeting or the result of metabolic rewiring [23, 89,90,91,92]