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Afterward, the membranes were incubated with the appropriate horseradish peroxidase-conjugated secondary antibody and immunoreactive bands were detected by a Fluorchem Imaging System upon staining with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA)

In the antioxidant defense system, Mn is part of superoxide dismutase (SOD)

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This may also be consistent with impaired mitochondrial biogenesis as described in circulating lymphocytes in chronic inflammatory conditions (45) and in patients with idiopathic ME/CFS ( Also, we detected a reduction in serine concentration, which was inversely associated with depression and anxiety scores in LC patients

, which also circumvents the need for derivatization (Vera et al., 2020)

LC-MSMS characterisation of curry leaf flavonols and antioxidant activity