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Pflugers Arch (2014) 466(6):111327

Dysregulation of PGC-1, a master regulator of mitochondrial metabolism, leads to increased neuronal vulnerability

They are CD8+, and their growth and differentiation may be regulated by CD4+ cells

Typically, E3s recognize substrates that are modified, often by phosphorylation (for more details, read Zheng & Shabek and Shabek & Zheng and Shabek & Ciechanover)