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The relative expression levels of randomly selected genes were calculated with the 2 CT method (Livak and Schmittgen, 2001)

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It is likely that all main isoenzymes present in the choroidal tissue during the early developmental period, namely GST mu1&2, GST alpha3&4, GST pi1 are sensitive to Nrf2 activation, as these isoenzymes are upregulated by inducers of the Nrf2 signaling pathway in the liver of adult rats [32]