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14 PCR amplification was performed in a final volume of 25 l of the reaction mixture containing 1.5 U of Taq DNA polymerase (Sibenzyme), about 1 g DNA of each patient's DNA, 0.25 M each primer, 250 M of dNTPs, 2 mM of MgCl 2 , 1 PCR buffer of the following composition: 67 mM Tris-HCl pH 8.8, 16.6 mM (NH 4 ) 2 SO 4 and 0.01% Tween-20

10.1016/j.chom.2023.01.011 387 SampsonT

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This finding supports the hypothesis that hyaluronic acid injections can not only improve symptoms but also protect cartilage and prevent further cartilage degradation (27)

On average, effects can last anywhere from a few weeks to a few months