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Cells were washed with PBS twice, for 3 min each and then incubated with 2 N HCl for 30 min at 37 C

GHK-Cu has the advantage of being a naturally occurring human peptide with no reported adverse effects

However, always adhere to the prescribed frequency

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Notes: Topical GHK-Cu should be applied post-washing in the morning, or otherwise in the evening at bedtime, to allow for maximum absorption

Vernoux T et al (2000) The ROOT MERISTEMLESS1 / CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of Cell Division during Postembryonic Root Development