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It does not appear toxic, does not accumulate in tissues, and has been well tolerated in trials

This premise is based on the notion that subzero temperatures in supercooled storage can substantially slow down metabolism to mitigate the metabolic storage lesion and thus extend storage time

Werner syndrome (WS), which is distinguished by severe metabolic dysfunction, dyslipidemia, early atherosclerosis, and insulin resistance diabetes, is thought to most closely mimic normal aging

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Additionally, obesity and IR both contribute to -cell dysfunction and apoptosis, mechanistically explained by glucotoxicity and increased immunogenicity (the accelerator hypothesis) or by -cell exhaustion due to increased metabolic demand (the overload hypothesis) [23]