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67 In line with a role of ATII in endothelial dysfunction and hypertension, numerous studies have now demonstrated that the therapy with angiotensin-converting enzyme inhibitors and AT-1 receptor blockers is associated with an improvements in endothelial responsiveness 68, 69, 70, 71, 72 (the existence of negative reports also needs to be acknowledged)

doi: 10.1038/nrrheum.2015.169 19 ZitvogelLGalluzziLKeppOSmythMJKroemerG

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Soft-agar clonogenicity assay Firstly, we mixed 3.5% low melting point agarose (#16520050, Thermo Fisher Scientific) and media (the volume ratio of agarose and media is 1: 5) to prepare the bottom soft-agar and then added it into 12-well plates quickly (1 mL soft-agar/well)