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Planta 199: 552-556

In comparison to the high specific ATP content, the cellular contents of the other nucleoside triphosphates are much lower in cultured astrocytes [66, 69], consistent with the cellular need of ATP as general energy currency for a broad range of cellular reactions (Table 1), while other nucleoside triphosphates are used in a more limited number of specific processes

4.4 Pyridoxine or vitamin B6 Pyridoxine or vitamin B6, is an essential water-soluble vitamin involved as cofactor in different metabolic pathways (94)

-GT activity is usually measured by kinetic spectrophotometric methods based on the work of Szasz, which are precise and inexpensive

Therefore, to functionally assess the cells ability to foster lipid biogenesis, we pulsed 67NR, 4T07, and 4T1 cells with 14 C-Glucose and other potential citrate and/or acetyl-CoA precursors (i.e., 14 C-Lactate, 14 C-Acetate, and 14 C-Glutamine) before recovering the radiolabeled lipid fraction