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The citric acid cycle involves three NAD+-dependent dehydrogenase reactions: isocitrate dehydrogenase, -ketoglutarate dehydrogenase, and malate dehydrogenase, each generating NADH while releasing carbon dioxide and regenerating oxaloacetate for continued cycle operation

Briefly, frozen liver aliquots were homogenized in Serine Assay Buffer in a Potter-Elvehjem homogenizer and centrifuged at 15000 g, for 10 min at 4C

The molecular mechanism underlying the relationship between OXPHOS and PDAC progression still requires in-depth study with bright prospects (Text Box S2)

Systematic review with meta-analysis: coffee consumption and the risk of gallstone disease

doi: 10.3389/fnut.2022.964948 225

Dendritic cells are crucial for maintenance of tertiary lymphoid structures in the lung of influenza virus-infected mice